The second-ranked Jayhawks could not beat the area on Wednesday and lost for the first time.
A 74-65 loss to Washington was the second straight game in which Kansas (7-1) fought a zonal defense.
Kansas entered as 23-point favorites against Washington at the Sprint Center in Kansas City, but a cold night of shooting against the area caused the Hawks' demise.
Coming out of a 16-point win over Syracuse in which Devonte Graham matched her career highs with 35 points, Kansas could not convert from deep into the Washington area, as it knocked down only five triples and Graham He remained at only three points in 1 of 8 shots.
While Kansas was not able to draw 3-pointers, Washington, who went in shooting 32 percent beyond the goal, hit nine treys led by Matisse Thybulle's five deep balls.
LaGerald Vick did his best to keep Kansas in the game, scoring 28 points with five rebounds and seven assists, but finally Graham's poor night cost the Hawks victory.
With Kansas in the loss column, Duke, Florida State, Miami, TCU, Villanova, Georgetown, Valparaiso, Arizona State and Mississippi State are the remaining undefeated teams in the DI basketball.