The Marlins let a nine-run lead disappear during a historically bad loss the day before.
The loss of Friday night hurts in a different way.
Typically, dominant closer Kyle Barraclough allowed a solo homer as wide receiver to Mark Reynolds in the bottom of the ninth inning while the Marlins fell 3-2 to the Nationals hosts.
The Marlins (36-54) lost their 13 th followed by the Nationals (44-43) marking their worst losing streak against an opponent in the history of the franchise.
For Barraclough, who was named National League Reliever of the Month for June and had a 20 2/3 scoreless scoring run until Monday's game against the Rays, it was his third loss of the season.
Reynolds' at-bat opened the ninth inning. Barraclough dropped behind 3-0 in Reynolds' count and then threw a strike. Reynolds then scored a 95.4 mph fastball from Barraclough and sent her 396 feet to the Marlins bullpen in left center field.
"Kyle has been so successful in getting ahead in the count and running his pitches," said Marlins manager Don Mattingly. . "He just stood behind and does not want to walk with him and it looks like [Reynolds] he got one in his wheelhouse."
He needed a quality start from his first position after his bullpen was again taxed in Thursday's Dan Straily debacle delivered by throwing 111 high-season pitches in six innings.
Straily had only one strikeout, but only allowed two runs on five hits and three walks.  "[Getting deep into games] That's always the goal," Straily said. "The way the pen was used the last two day cuple, but every time you take the ball you want to throw nine innings"
The Nationals almost broke a 2-2 draw in the sixth when Wilmer Difo sent a ball in the right center garden gap that Adam Eaton would have scored if he had landed. But Cameron Maybin tracked him down and made a spectacular dive catch to finish the entry.
"Unreal," Straily said about the capture. "I was bothering myself for having left a double in the gap and he comes in and catches it, it was amazing."
The Marlins ran to the field of Monseñor Pace, former student of Gio González, up to 114 in five innings. Gonzalez allowed eight hits and walked four, but struck out four and limited the Marlins to just two runs.
The Marlins connected three double plays and the Nationals converted another in the first inning when Gonzalez struck Realmuto and Brian Anderson was sent off in the second by receiver Spencer Kieboom.
The Marlins loaded the bases in the fourth and were left empty when Cameron Maybin landed in a double play. They reloaded in the fifth and scored a race when Gonzalez received Martin Prado. Gonzalez then struck out Justin Bour and Garrett Cooper, the last two batters he faced.
"These were once again the kind of games that give us the chance to win and Dan led the charge." Mattingly said. "We did a good job with Gio and we just could not break through."
Drew Steckenrider extended his run of innings without allowing runs to 18 2/3, but almost broke in the eighth when Anthony Rendon and Bryce Harper walked and gave a single to Matt Adams to open the frame. Steckenrider then made Adam Eaton fly to the left and Cooper made a strong throw to keep the runner in third position. Wilmer Difo then appeared on shortstop and Daniel Murphy appeared in the center.
Cooper played in his first game since April 1 and was 2 for 3, also making some tough catches in left field.
"Going back and putting your feet under you and being back in this environment was good," Cooper said. "The first fly in the first inning was good to drive him crazy."