Meta-DNA Structures Change the World of DNA Nanotechnology

Model and transmission electron microscopy (TEM) images of various 3D polyhedra that were created by combining self-linked triangular M-DNA and rectangular M-DNA. From left to right: a tetrahedron, triangular bisector, octagonal, pentagonal binomial, triangular prism, rectangular prism, pentagonal and hexagonal prism. Sincerely: Hao Yan

A team of scientists from ASU and Shanghai Jiao Tong University (SJTU), led by Hao Yan, ASU’s Milton Glick Professor in School of Molecular Sciences, and director of ASU Biodesign Institute’s Center for Moleole Design and Biomimetics, have just added scientists Has announced the creation of a new type of meta-DNA structures that will open the fields of optoelectronics (including information storage and encryption) as well as synthetic psychology.

This research was published today Nature chemistry– Meta-DNA can transform the self-assembly concept into the microscopic world of fully structural DNA nanotechnology.

It is common knowledge that Watson-Crick base-pairing and the predictive nature of the structural features of DNA have allowed DNA to be used as a versatile building block for engineered sophisticated nanoscale structures and devices.

“A milestone in DNA technology was certainly the invention of DNA origami, where a long single-stranded DNA (ssDNA) is transformed into designated shapes with the help of hundreds of short DNA staple strands.” “However it has been challenging to assemble large (micron to millimeter) DNA architectures that have until recently limited the use of DNA origami.” The new micron-sized structures are on the order of width of a human hair that is 1000 times larger than the original DNA nanostructure.

Since the cover is covered Science In 2011 with their elegant DNA origami nanostructures, Yan and colleagues have been continuously working to solve complex human problems, capitalizing on inspiration from nature.

“In this current research we have developed a versatile” meta-DNA “(M-DNA) strategy that allows various sub-micrometers to self-assemble micrometer-sized DNA structures into how simple miniature DNAs self-assemble. Assembles. Nanoscale level, “Yan said.

The group demonstrated that a 6-helix bundle DNA origami nanostructure at the sub-micrometer scale (meta-DNA) could be used as a magnified analog of single-stranded DNA (ssDNA), and that two meta-DNAs Supplements are “meta-“. Base pairs “can form double helicopters with programmed position and helical pitches.

Using meta-DNA building blocks, they have constructed a series of sub-micrometers for DNA architecture on a micro-scale scale, including meta-multi-arm junctions, 3-D polyhedrons, and various 2-D / 3-D. Latitude included. They performed a hierarchical strand-displacement reaction on meta-DNA to transfer the dynamic features of DNA to meta-DNA.

With the help of Assistant Professor Petr Sulak (SMS), he used a coarse-grained computational model of DNA to simulate the double-stranded M-DNA structure and to understand the different yields of left-right and right-handed structures. For which were obtained.

In addition, by changing the local flexibility and their interactions of individual M-DNA, they were able to create a series of sub-micrometer or micron-scale DNA structures ranging from 1D to 3-D, including a variety of geometric shapes. . Meta-junction, meta-double crossover tile (M-DX), tetrahedron, octahedron, prism and six types of closely packed latite.

In the future, more complex circuits, molecular motors and nanodevises can be rationally designed using M-DNA and can be used in applications related to biosensing and molecular calculations. This research will create dynamic micron-scale DNA structures, which are reusable upon stimulation, are significantly more possible.

The authors hypothesize that the introduction of this M-DNA strategy will change DNA nanotechnology from the nanometer to the microscopic scale. This will create a series of complex static and dynamic structures in sub-micrometer and micron-scale that will enable many new applications.

For example, these structures can be used as a scaffold to pattern complex functional components that are larger and more complex than previously possible. This finding may lead to more sophisticated and complex behaviors that combine cell or cellular components with distinct M-DNA based hierarchical strand displacement reactions.

Using DNA origami to construct future nanodevices

more information:
Meta-DNA structures, Nature chemistry (2020). DOI: 10.1038 / s41557-020-0539-8,

Provided by Arizona State University

Quotes: A New Twist on DNA Origami: Meta-DNA Structures Transform the World of DNA Nanotechnology (2020, September 7) Retrieved nano world.html from 7 September 2020

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