Kovid 19 Vaccine Update: Novel vaccine candidate shows promise against Kovid-19, studies. world News


Washington: According to a study conducted on mice, a particular vaccine that promotes the production of specific proteins may be effective against the novel coronovirus of Kovid-19.
Scientists at Ohio State University in the US manipulated a natural cellular process to ramp up levels of two proteins used by viruses to infect other cells, pack protein-enhancing instructions into nanoparticles, and transfer them to mice. Injected into.
Within a month, the mice had developed antibodies against the SARS-CoV-2 virus, according to the study published Wednesday in the journal Advanced Materials.
Researchers include altering specific sequences of messenger RNA, molecules that convert genetic information into functional proteins, the researchers said.
Although these sequences have not been translated into proteins, the researchers altered their structures to promote higher-level normal levels of the protein.
The sequences are known as the untranslated region or UTR.
Although Phase 3 clinical trials of the phase-track Kovid-19 vaccine are in progress, Yizhou Dong, an associate professor at Ohio State University, said the stage of his lab offers a potential alternative.
“If current vaccines work well, that’s amazing. If the field needs it, it’s an option. It works as a vaccine, and we can grow it very quickly,” Dong he said.
“For now, this is a proof of concept – we have demonstrated that we can adapt a sequence of messenger RNAs to improve protein production, produce antigens, and induce antibodies against those specific antigens, ” They said.
The method is specific to cruc vaccine development: using snippets of a pathogen’s structure to produce an antigen – the foreign substance that triggers an appropriate immune response – and find a safe way to introduce it into the body is.
However, the technology takes antigen design to a new level using the messenger RNA UTR, Dong said.
His laboratory worked with two UTRs that book the initiation and termination of protein assembly, acting as regulators of that process and influencing how the resulting protein interacts with others.
The UTRs themselves are strands of nucleotides, the molecules that compose RNA and DNA, the researchers said.
“For our application we tried to optimize the UTRS to improve the protein production process. We wanted to produce as much protein as possible – so we could deliver a small dose of messenger RNA that would allow antibodies against the virus Produces enough antigen to induce, ”Dong said.
The team experimented with two potential antigens that the novel coronavirus is known to cause infection: a spike protein on its surface and a receptor binding domain.
The domain spike is a component of the protein that the virus uses to make its way into the host cells – an essential step to make copies of itself.
Researchers said that both are used in other SARS-CoV-2 vaccine candidates.
After manipulating the messenger RNA for these two proteins, the team encapsulated them in lipid nanoparticles already developed in Dong’s laboratory.
He injected mice with the experimental vaccine and gave them a booster two weeks later.
One month after the first injection, immune cells in mice had taken the antigen of two proteins and developed antibodies against them.
“It takes some time for the immune system to process the antigen and the cells produce antibodies. In this study, we detected antibodies after 30 days,” said Dong.
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